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KMID : 1141020180180010067
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Journal of Applied Oriental Medicine
2018 Volume.18 No. 1 p.67 ~ p.74
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Comparison of Antioxidative Activity of Ethanol Extracts of Rehmanniae Radix Crudus Depending on Storage Period
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Bae Hye-Kyung
Lee Jin-A
Roh Seong-Soo
Seo Bu-Il
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Abstract
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Objectives : This study was performed to investigate the antioxidant activity of 30% ethanol extracts of Rehmanniae Radix Crudus depending on storage period. This study was performed to investigate the antioxidant activity of 30% ethanol extracts of Rehmanniae Radix Crudus depending on storage period.
Methods : Rehmanniae Radix Crudus (RC) depending on storage period extract were prepared using 30% ethanol. The RC used immediately was R0, and the RC stored at 2 month was R2. The total polyphenol and total flavonoid contents were analyzed. And 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) scavenging activity assays were carried out to determine the antioxidant activities.
Results : Higher total polyphenol contents were found in the R0 extract (3.69±0.02 ㎎/g) than in the R2 extract (3.62±0.08 ㎎/g). The flavonoid contents in R0 and R2 extract were 1.50±0.07 ㎎/g, 3.76±0.40 ㎎/g respectively. Also, the DPPH free radical scavenging activity in R0 and R2 extract were IC50=524.43±4.14 ㎍/㎖, IC50=570.17±1.43 ㎍/㎖ respectively, and the ABTS free radical scavenging activit were IC50=700.54±4.68 ㎍/㎖, IC50=789.34±3.20 ㎍/㎖ respectively.
Conclusions : These results suggest that R0 extract is superior to R2 extract in anti-oxidant capacity thus R0 can be applied in variable antioxidative products.
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KEYWORD
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Rehmanniae Radix Crudus, Antioxidant, depending on storage
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